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Journal: Pharmacology Research & Perspectives
Article Title: Apigenin Protects H9c2 Cells Against Oxygen–Glucose Deprivation/Reperfusion Injury by Regulating Autophagy via the HIF ‐1α/miR‐20a Axis
doi: 10.1002/prp2.70224
Figure Lengend Snippet: Effects of CA‐5f on OGD/R‐induced viability, SOD activity, LDH, MDA, GSH levels, and autophagy marker protein expression in H9c2 cells. (A) The viability of H9c2 cells treated with CA‐5f (10 μM) was assessed by MTT assay. (B, C) LDH leakage, SOD activity levels in H9c2 cells. (D) Cellular ROS levels after OGD/R and CA‐5f (10 μM) application (scale bar: 100 μm). (E, F) Levels of MDA and GSH in different treatment groups. (G) The expression of autophagy‐related proteins (LC3II/I, p62) was detected by Western blot. ( n = 5). * p < 0.05.
Article Snippet: HIF‐1α inhibitor Guanylyl cyclase activator (YC‐1) (BIOFOUNT, China),
Techniques: Activity Assay, Marker, Expressing, MTT Assay, Western Blot
Journal: Annals of Medicine
Article Title: PPAR-mediated reduction of lipid accumulation in hepatocytes involves the autophagy-lysosome-mitochondrion axis
doi: 10.1080/07853890.2025.2497112
Figure Lengend Snippet: Effect of selective inhibitors on lipid accumulation induced by oleate and palmitate. Lipid accumulation assessed as ORO-positive area in cells treated with oleate and palmitate (O/P) for 48h in the presence or absence of the PPAR agonists fenofibric acid (FA), pioglitazone (PGZ), seladelpar (SEL) or saroglitazar (SAR). Cells were pre-treated with CA-5f (Panel A), lalistat 2 (Panel B) or etomoxir (Panel C). Data are expressed as fold of untreated cells, mean ± SD, n = 3. * p < 0.05 vs O/P. Representative images are shown in Panel D (scale bar 100 μm).
Article Snippet: In particular, cells were pre-treated for 6h with (i) the inhibitor of the
Techniques: